canine-specific commercial elisa kits  (FineTest Biotech Inc)

Journal: PLoS ONE

Article Title: The effect of naltrexone as a carboplatin chemotherapy-associated drug on the immune response, quality of life and survival of dogs with mammary carcinoma

doi: 10.1371/journal.pone.0204830

Figure Lengend Snippet: The results are divided into before and after the proposed treatment and subcategorized by the absence (-) or presence (+) of metastasis. Beta-endorphin concentrations for each sample (in pg/mL) were determined by ELISA (A). Met-enkephalin concentrations for each sample were determined by ELISA and calculated in ng/mL (B). *Significant differences at p < 0.05, according to the Kruskal-Wallis statistical test.

Article Snippet: Serum samples were obtained from blood collected 50 minutes before and six months after mastectomy and were used for beta-endorphin and met-enkephalin quantification, employing commercial canine-specific ELISA kits (MyBioSource, San Diego, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Associations between serum leptin levels, hyperlipidemia, and cholelithiasis in dogs

doi: 10.1371/journal.pone.0187315

Figure Lengend Snippet: (A) In dogs with cholelithiasis (n = 34), serum leptin levels were significantly higher than those determined in healthy dogs (n = 28). (B) Serum leptin levels were significantly higher in cholelithiasis patients that underwent cholecystectomy compared with those in patients that did not undergo surgery. Horizontal bars in scatter plots indicate median values. ** p < 0.01, *** p < 0.001 between groups.

Article Snippet: Serum leptin concentrations in all samples were analyzed in duplicate using a commercial canine-specific leptin sandwich ELISA kit (Canine Leptin ELISA, Millipore, Billerica, MA, USA) according to the manufacturer’s protocol.

Techniques:

Journal: PLoS ONE

Article Title: Associations between serum leptin levels, hyperlipidemia, and cholelithiasis in dogs

doi: 10.1371/journal.pone.0187315

Figure Lengend Snippet: The cholelithiasis group (n = 34) was divided according to the presence (n = 7) or absence (n = 27) of diabetes mellitus; the concurrence of diabetes with cholelithiasis had no significant effect on serum (A) leptin or (B) insulin levels. Horizontal bars in scatter plots indicate median values. ** p < 0.01, *** p < 0.001 between groups.

Article Snippet: Serum leptin concentrations in all samples were analyzed in duplicate using a commercial canine-specific leptin sandwich ELISA kit (Canine Leptin ELISA, Millipore, Billerica, MA, USA) according to the manufacturer’s protocol.

Techniques:

Journal: PLoS ONE

Article Title: Associations between serum leptin levels, hyperlipidemia, and cholelithiasis in dogs

doi: 10.1371/journal.pone.0187315

Figure Lengend Snippet: The mRNA expression levels of (A) leptin and (B) leptin receptor were significantly higher in patients with cholelithiasis (n = 10) than in the controls (n = 10). Columns indicate mean values, and vertical error bars represent standard deviation. ** p < 0.01, *** p < 0.001 between groups.

Article Snippet: Serum leptin concentrations in all samples were analyzed in duplicate using a commercial canine-specific leptin sandwich ELISA kit (Canine Leptin ELISA, Millipore, Billerica, MA, USA) according to the manufacturer’s protocol.

Techniques: Expressing, Standard Deviation

Journal: PLoS ONE

Article Title: Associations between serum leptin levels, hyperlipidemia, and cholelithiasis in dogs

doi: 10.1371/journal.pone.0187315

Figure Lengend Snippet: A significant positive correlation between serum leptin and total cholesterol levels was observed in both controls and patients.

Article Snippet: Serum leptin concentrations in all samples were analyzed in duplicate using a commercial canine-specific leptin sandwich ELISA kit (Canine Leptin ELISA, Millipore, Billerica, MA, USA) according to the manufacturer’s protocol.

Techniques:

Journal: PLoS ONE

Article Title: Associations between serum leptin levels, hyperlipidemia, and cholelithiasis in dogs

doi: 10.1371/journal.pone.0187315

Figure Lengend Snippet: A significant positive correlation between serum leptin and triglyceride levels was observed in both controls and patients.

Article Snippet: Serum leptin concentrations in all samples were analyzed in duplicate using a commercial canine-specific leptin sandwich ELISA kit (Canine Leptin ELISA, Millipore, Billerica, MA, USA) according to the manufacturer’s protocol.

Techniques:

Journal: The Journal of Veterinary Medical Science

Article Title: CD4 + T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease

doi: 10.1292/jvms.13-0008

Figure Lengend Snippet: Sequences of oligonucleotide primers used for quantitative real-time PCR

Article Snippet: Protein levels were determined using commercial ELISA kits specific for canine IL-17A (USCN Life Science, Wuhan, China, 100 μl of supernatants/well), IFN-γ and IL-10 (R&D systems, Minneapolis, MN, U.S.A., 50 μl supernatant/well).

Techniques: